DIA-REAL-AVIAN INFLUENZA KIT FOR ASSAYING RNA OF AVIAN INFLUENZA A VIRUS (H5N1) BY ONE-STAGE REAL-TIME REVERSE-TRANSCRIPTASE POLYMERASE CHAIN REACTION

摘要

DIA-REAL-AVIAN INFLUENZA kit for assaying RNA of avian influenza A virus (H5N1) by one-stage real-time reverse-transcriptase polymerase chain reaction. cDNA fragments of three genes of avian influenza virus (highly conservative portions of M, N1 and H5 genes) are synthesized on virion RNA template. The fragments are then amplified in polymerase chain reaction. The original oligonucleotide primers and TaqMan probes labeled by fluorescent stains are used for the specific detection of virion RNA of avian influenza A virus (H5N1) in real-time RT-PCR. The kit also contains the reagents for isolating viral RNA: «QIAamp Viral RNA Mini Kit» and "PureArtTM Viral RNA Mini Kit".