发明名称 Chimeric gene constructs for generation of fluorescent transgenic ornamental fish
摘要 Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed, respectively, were isolated and ligated to the 5' end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultra-violet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patters, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.
申请公布号 US7135613(B1) 申请公布日期 2006.11.14
申请号 US20010913898 申请日期 2001.11.27
申请人 THE NATIONAL UNIVERSITY OF SINGAPORE 发明人 GONG ZHIYUAN;HE JIANGYAN;JU BENSHENG;LAM TOONG JIN;XU YANFEI;YAN TIE
分类号 A01K67/00;A01K67/027;C07H21/02;C07H21/04;C07K14/435;C12N5/16;C12N15/12;C12N15/85;C12Q1/48;C12Q1/66;G01N33/18;G01N33/74 主分类号 A01K67/00
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