发明名称 |
Synthesis of polynucleotides using combined oxidation/deprotection chemistry |
摘要 |
A method of synthesizing a polynucleotide which can, for example, be used during fabrication of an array. A second nucleoside is coupled to a first nucleoside through a phosphite linkage, with the second nucleoside having a hydroxyl protecting group that is a non-carbonate protecting group. The product of the foregoing step is exposed to a composition which both oxidizes the formed phosphite to a phosphate and deprotects the protected hydroxyl of the coupled nucleoside. The method has particular application to fabricating an addressable array of polynucleotides on a substrate which carries substrate bound moieties each with a hydroxyl group.
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申请公布号 |
US7135565(B2) |
申请公布日期 |
2006.11.14 |
申请号 |
US20010916369 |
申请日期 |
2001.07.27 |
申请人 |
UNIVERSITY OF COLORADO |
发明人 |
DELLINGER DOUGLAS J.;PERBOST MICHAEL G. M.;CARUTHERS MARVIN H.;BETLEY JASON R. |
分类号 |
C07H21/00;A01N43/04;A61K31/70;C07H21/02;C07H21/04 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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