发明名称 |
STRAIN OF BACTERIUM Glacial ice bacterium I AS PRODUCER OF RESTRICTION ENDONUCLEASE Gla I |
摘要 |
FIELD: biotechnology, genetic engineering, biochemistry, enzymes. ^ SUBSTANCE: invention relates to preparing a novel strain of microorganism used for isolation of the novel restriction endonuclease Gla I. The strain Glacial ice bacterium I is isolated from a reservoir as result of the search of producers of restrictases and provides preparing the restriction endonuclease Gla I recognizing and cleaving both chains of DNA nucleotide sequence comprising C5-methylcytosine bases in the recognition site 5'-GCGC-3'. Invention provides preparing the yield of enzyme 10 U/g of crude biomass with specific activity of the end enzyme 200 U/ml. This novel restriction endonuclease can be used for detection of methylated DNA. ^ EFFECT: valuable biological and biochemical properties of enzyme. ^ 1 tbl, 3 dwg, 4 ex |
申请公布号 |
RU2287012(C1) |
申请公布日期 |
2006.11.10 |
申请号 |
RU20050111829 |
申请日期 |
2005.04.20 |
申请人 |
OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOST'JU "SIBEHNZAJM" |
发明人 |
CHERNUKHIN VALERIJ ALEKSEEVICH;NAJAKSHINA TAT'JANA NIKOLAEVNA;MEZENTSEVA NINA VLADIMIROVNA;TOMILOVA JULIJA EHDUARDOVNA;DEGTJAREV SERGEJ KHARITONOVICH;DEDKOV VLADIMIR SERGEEVICH |
分类号 |
C12N1/21;C12N9/14;C12R1/01 |
主分类号 |
C12N1/21 |
代理机构 |
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主权项 |
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地址 |
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