СПОСОБ КРИОКОНСЕРВАЦИИ ГРЕБНЕВИКА, НАПРИМЕР BEROEOVATA

摘要

FIELD: biotechnology, method for cryoconservation of biological cells for prolonged storage. ^ SUBSTANCE: claimed method includes selection of fertilized dog's tail Beroe ovata eggs in step of 2-4 blastomeres. Then eggs are placed in barrier solution, frozen by dipping in liquid nitrogen followed by storage therein. Further eggs are defrosted followed by washing thereof in washing solution. Sea water used as physiological medium for barrier and washing solutions is preliminary sterilized by filtration through membrane filter. ^ EFFECT: increased yield of non-damaged dog's tail eggs being capable of further development. ^ 2 tbl, 8 ex