| 摘要 |
FIELD: production of iron-containing biologically active food supplement useful in prophylaxis of iron-deficit condition in human. ^ SUBSTANCE: claimed method includes Andara broughtoni haemolymph frosting, defrosting, centrifugation, filtering on membrane with pore size of 0.2 mum. Further obtained product is concentrated and washed from contaminants with water in ultrafiltration apparatus with membrane, passing globular proteins with molar mass of 300 kDa or less to produce oxyhemoglobin. Then proteolytic enzyme is added to oxyhaemoglobin in oxyhaemoglobin/enzyme ratio of 100:1-2. Mixture is incubated for 4-5 h at 29-30°C. Obtained haemoprotein is washed with distilled water to remove low molecular hydrolysis products and concentrated in ultrafiltration apparatus with membrane of 30 kDa. Claimed food supplement contains bivalent iron in amount of 3-5 % based on dry material mass that is higher by 15-20 times in contrast to supplement obtained from animal blood. ^ EFFECT: bioactive food supplement with sufficiently increased bivalent iron content. ^ 2 cl, 1 dwg, 1 tbl, 3 ex |
