发明名称 |
Materials and methods for detection of nucleic acids |
摘要 |
Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample. |
申请公布号 |
US2006252091(A1) |
申请公布日期 |
2006.11.09 |
申请号 |
US20060486841 |
申请日期 |
2006.07.14 |
申请人 |
ERAGEN BIOSCIENCES, INC. |
发明人 |
MARSHALL DAVID J.;PRUDENT JAMES R.;SHERRILL CHRISTOPHER B.;SHAPIRO GIDEON;GRENIER JENNIFER K.;RICHMOND CRAIG S.;JURCZYK SIMONA;PTACIN JEROD L. |
分类号 |
C12Q1/68;G01N21/64;C12N15/09;C12Q1/48;G01N21/78;G01N33/483;G01N33/53;G01N33/566;G01N33/58 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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地址 |
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