发明名称 |
METHOD FOR SEARCHING GENOME-WIDE RESTRICTION ENZYME REACTION SITE OR VARIATION OR MODIFICATION SITE WHICH IS CARRIED OUT WITH TRACE AMOUNT OF CELL AND KIT THEREFOR |
摘要 |
PROBLEM TO BE SOLVED: To provide a method for detecting a restriction enzyme reaction site or a variation site in a DNA, which is carried out with a trace amount of a DNA and does not require a specific apparatus and tool. SOLUTION: The method for detecting a restriction enzyme reaction site or a variation or modification site in a genome DNA comprises (i) obtaining a genome DNA from cells to be examined, treating the genome DNA with a restriction enzyme or a site-specific enzyme and performing a long PCR using the genome DNA treated with the enzyme as a template, (ii) carrying out a short PCR using an amplification product as a template and then (iii) comparing the results of the short PCR of samples treated with and without the enzyme. (iv) When a variation is detected, the result of normal cells obtained by performing the processes (i)-(iii) is compared with the result of cells to be examined. COPYRIGHT: (C)2007,JPO&INPIT
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申请公布号 |
JP2006304709(A) |
申请公布日期 |
2006.11.09 |
申请号 |
JP20050132390 |
申请日期 |
2005.04.28 |
申请人 |
INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH |
发明人 |
HAMADA TAKESHI;ASAHARA TAKAYUKI |
分类号 |
C12Q1/68;C12N15/09;C12Q1/42 |
主分类号 |
C12Q1/68 |
代理机构 |
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