| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method so that a PNA probe sufficiently reacts with an objective gene and the mutation of DNA of the objective gene is accurately detected even in a specimen in which genes of not the object of detection, present in a high concentration. <P>SOLUTION: This PCR method is provided by performing an amplification by using a primer set containing a base sequence of a normal k-ras of which objective gene is bonded with the PNA, as a part and another primer set without containing the above base sequence; in the case that the k-ras gene is mutated, then the PNA can not bind with it, and the mutated gene is amplified by the PCR; if the k-ras gene is not mutated and is normal, since the PNA can bind with it, and on the contrary, the primer having an overlap in part with the k-ras gene, can not bind with it, the subject gene can not be amplified by the PCR; and therefore by the amplification using the primer set without containing the base sequence of the k-ras, the PNA probe sufficiently reacts even in the specimen in which the genes of not the object of the detection, present in the high concentration, to prevent an erroneous judgement. <P>COPYRIGHT: (C)2007,JPO&INPIT |
