| 摘要 |
PROBLEM TO BE SOLVED: To provide a labeled nucleotide terminator compound usable in a primer extension reaction without necessitating a large excess of the compound relative to a non-labeled extensible nucleotide and forming a uniform peak height distribution in Sanger-type sequence determination reaction. SOLUTION: The nucleoside/nucleotide compound has the following structure: NUC-L-S-LB/LG, wherein NUC is a nucleoside/nucleotide having a nucleobase portion B; L is a rigid linkage; S is a spacer; LB/LG is a member of a linkage pair or a label; NUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine. COPYRIGHT: (C)2007,JPO&INPIT
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