发明名称 |
Isolation of a dimer di-gallate, a potent endothelium-dependent vasorelaxing compound |
摘要 |
Certain proanthocyanidins found in various wines, grape juice, and other plant extracts exhibit endothelium-dependent vasorelaxing activity that involves increased nitric oxide production by endothelial cells. The smallest procyanidins (PCs) possessing substantial EDR activity were isolated from grape seeds. A dimer di-gallate (epicatechin-galloyl-epicatechin-gallate) was purified from concord grape seed extracts by Toyopearl TSK-40 gel chromatography, HPLC and electrospray FTMS. Further characterization was achieved using tannase treatment and acid thiolysis. This compound had an EC50 for vasorelaxation of 0.67±0.04 mug/ml when tested for endothelium-dependent relaxing activity in phenylephrine pre-contracted rat aortic rings.
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申请公布号 |
US7132446(B1) |
申请公布日期 |
2006.11.07 |
申请号 |
US20030605787 |
申请日期 |
2003.10.27 |
申请人 |
UNIVERSITY OF SOUTH FLORIDA |
发明人 |
FITZPATRICK DAVID F.;O'MALLEY REBECCA |
分类号 |
A61K31/35 |
主分类号 |
A61K31/35 |
代理机构 |
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