发明名称 |
Compounds and methods for inhibiting hepatitis C virus replication |
摘要 |
The inventors have discovered that an ATPase-deficient dominant-negative mutant NS3 protein of hepatitis C virus inhibits activity of the wild-type NS3 protein and inhibits replication of hepatitis C virus (HCV). The solved crystal structure of a multi-enzyme NS3 complex on a DNA substrate is also provided. The inventors have tested a peptide matching the sequence of a portion of NS3 that interacts with another NS3 molecule for inhibiting HCV replication. The peptide inhibits HCV replication. Accordingly, the invention provides a method of inhibiting HCV replication in cells infected with HCV involving transforming the cells with a vector expressing a dominant-negative mutant NS3 gene. The invention also provides a method of inhibiting HCV replication in cells infected with HCV involving administering to the cells a dominant-negative mutant NS3 protein. The invention also provides peptides and agents that inhibit HCV replication and methods of identifying agents that inhibit HCV replication. |
申请公布号 |
US2006246421(A1) |
申请公布日期 |
2006.11.02 |
申请号 |
US20050119587 |
申请日期 |
2005.05.01 |
申请人 |
THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS |
发明人 |
RANEY KEVIN D.;CAMERON CRAIG E.;DAVE BHUVANESH;SAKON JOSHUA;LU JEFF Z.;MACKINTOSH SAMUEL G.;JENNINGS THOMAS A. |
分类号 |
C12Q1/70;A61K38/16;C12N15/86;G06F19/00 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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