| 摘要 |
In a process for the preparation of orotidine-(4(6) - carboxy - uracil - N - riboside), an artificially induced biochemical mutant strain of Bacillus subtilis having the capacity to produce orotidine extracellularly in a medium requiring uracil, uridine or uridylic acid as growth promoting agent, is aerobically cultured in an aqueous medium containing a source of assimilable carbon, a source of assimilable nitrogen, one or more inorganic salts and an organic growth promoting agent, including a source of uracil, uridine or uridylic acid, at a temperature between 25 DEG and 40 DEG C. and at a pH of between 5.0 and 8.5 until orotidine is accumulated in said medium. The product is extracted from the medium by absorption on an anion exchange resin.
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