METHOD FOR PORCINE CIRCOVIRUS PRODUCTION AND ASSAYS FOR MONITORING PRODUCTION

摘要

The present invention provides methods for the determination of the viral titer of a culture of host animal host cells infected with a circovirus. The FACS-based methods of the invention may include determining the viability of the host cells in a cell culture medium supernatant and of those cells that remain adherent to a solid support. Detecting and measuring the percentage o f cells that expressed the viral antigens ORFl and 0RF2 may determine the vira l load of the cultured host cells. The yield of the virus may be established b y the detection and measurement of both antigens in supernatant cells, for example (5) to (7) days from when the host cells are transferred to a serum free medium. The methods of the invention may yield rapid quantitative data. This allows the repeated in-process monitoring of the viral production throughout the incubation period, and ready selection of the most appropriat e harvesting point.