METHOD OF SYNTHESIZING 2-DEOXY-SCYLLO-INOSOSE BY MODIFIED STRAIN OF E. COLI, METHOD OF PURIFYING THE SAME AND OBTAINED 2-DEOXY-SCYLLO-INOSOSE

摘要

A transformant is prepared by introducing at least one kind of gene expression cassette comprising a gene involved in 2-deoxy-scyllo-inosose synthesis into E. coli as a host cell. Then, using this transformant, 2-deoxy-scyllo-inosose is synthesized from D-glucose, an oligosaccharide, a polysaccharide, a starch or rice bran. A culture medium containing 2-deoxy-scyllo-inosose is treated with a mixed-bed column comprising a hydrogen ion type strong acidic cation exchange resin and an organic acid ion type basic anion exchange resin.