摘要 |
<p>The present invention provides a method for isolating and purifying immuno-modulating polypeptide from cow placenta, which is characterized by using the steps of anion-exchange chromatography, gel exclusion chromatography and reverse-phase high performance liquid chromatography to isolate and purify immuno-modulating polypeptide from cow placenta, identifying its activity of stimulating lymphocyte proliferation in vitro by MTT method, then determining its molecular weight by MALDI-TOF-MS, its isoelectric point by CIEF and its amino acid sequence with analyzer for protein sequencing. Since the obtained immuno-modulating polypeptide by the method according to the present invention has more than 90% purity, its bioactivity can reach medicinal standards.</p> |
申请人 |
SHI JIA ZHUANG SANLU GROUP CO., LTD;WANG, YULIANG;SHENG, QINGHAI;FANG, XINPING;XIA, WENSHUI;ZHAI, HONGMEI;WEI, PENG |
发明人 |
WANG, YULIANG;SHENG, QINGHAI;FANG, XINPING;XIA, WENSHUI;ZHAI, HONGMEI;WEI, PENG |