СПОСОБ ОЦЕНКИ СПЕЦИФИЧЕСКОЙ АКТИВНОСТИ БАКТЕРИОФАГОВ

摘要

FIELD: analytical methods in microbiology. ^ SUBSTANCE: substance of the method resides in performing, under human embryo cell culture monolayer conditions, common 2-h culturing of test strains of microorganisms with suitable therapeutical bacteriophage or multifunctional (polyvalent) bacteriophage, which includes a phage corresponding to test culture. Specific activity of bacteriophage is evaluated from degree of adhesion of test strain of microorganism to monolayer cells compared to control (cell monolayer and test strain without bacteriophage). ^ EFFECT: speeded up determination and enabled evaluation of therapeutical bacteriophage due to approaching evaluation conditions to microorganism conditions. ^ 2 tbl