发明名称 |
TEMPERATURE-TRIGGERED IMMOBILIZATION AND PURIFICATION OF ANTIBODIES |
摘要 |
<p>This invention provides compositions and methods for capture and purification of antibodies. Protein sequences with inverted solubility temperature profiles are fused to antibody-binding proteins. Fusions of elastin-like peptides (ELPs) and protein G and/or protein L can be readily purified and employed to capture and purify immunoglobulins. To avoid the drawbacks of conventional affinity chromatography, ELP fusion proteins consisting of an elastin-like polypeptide made up of the pentapeptide VPGVG repeating units and Ig-binding ligands, protein A, protein G, protein L, and hybrid ligands such as protein LG are used for immobilization and purification of monoclonal or polyclonal antibodies from a material containing these antibodies by simple temperature transition cycling.</p> |
申请公布号 |
WO2006110292(A2) |
申请公布日期 |
2006.10.19 |
申请号 |
WO2006US10801 |
申请日期 |
2006.03.24 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;KIM, JAE-YOUNG;MULCHANDANI, ASHOK;CHEN, WILFRED |
发明人 |
KIM, JAE-YOUNG;MULCHANDANI, ASHOK;CHEN, WILFRED |
分类号 |
C07K16/18;C12P21/06 |
主分类号 |
C07K16/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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