COMPOSITIONS AND METHODS FOR TERMINATING A SEQUENCING REACTION AT A SPECIFIC LOCATION IN A TARGET DNA TEMPLATE

摘要

Compositions and methods for sequencing a template polynucleotide comprising a sequence of interest are provided herein. The compositions and methods employ at least one blocking probe that is designed to bind in a sequence-specific manner to a blocking sequence such that primer extension beyond the site where the blocking probe binds is reduced or prevented.