Methods for producing transgenic cotton plants using chilled apical shoot tips

摘要

This invention relates to methods for transforming cotton using isolated shoot apical tips of seedlings. The apical shoot tips of cotton seedlings are isolated and chilled at a low temperature to slow the metabolic activity of the isolated cells, and then inoculated with a transforming agent carrying a desired gene. The invention also provides a method of regenerating transformed cotton plants with a desired trait from transformed apical shoot tips.