Detecting potato virus Y responsible for necrosis, comprises extraction of nucleic acids from plant and amplification by reverse transcriptase-polymerase chain reaction from RNA viral region to detect polymorphisms

摘要

Detection of the presence or absence of potato virus Y (PVY) virus responsible for necrosis comprising extraction of nucleic acids from plant, amplification by RT-PCR of a region of viral RNA comprising codons 738 and 757 (SEQ ID No. 1 and 3) corresponding to amino acids 400 and 419, respectively, of the HC-Pro protein (SEQ ID No. 2 and 4), and detection of the presence or absence of mutations Arg/Lys400 and Asp/Glu419, where the detection of at least one of the mutations is an indication of a virulent strain of PVY virus in plants of the Solanaceae family. Independent claims are included for: (1) a process of medical selection of seedlings of Solanaceae family; (2) a detection kit; (3) a probe/collection of probes; (4) a primer/collection primers; and (5) a batch of seeds, seedlings and/or plants of Solanaceae family.