| 摘要 |
The invention relates to a method and device for identifying luminescent molecules according to the fluorescence correlation spectroscopy method. The inventive method comprising the following steps: a) preparing a sample ( 12 ) containing luminescent molecules; b) illuminating the sample ( 12 ) using an optical excitation device ( 2,4,6,8 ) comprising at least one light source, at least one, in particular, diffractive optical element ( 7 ) for splitting light passing therethrough into multiple beams, and a focusing optics ( 8 ) for focusing multiple light beams passing therethrough into multiple confocal volume elements: c) capturing emission radiation from the multiple confocal volume elements by means of a locally resolving sensor matrix arrangement ( 20 ), whereby the sensor matrix arrangement is a sensor matrix, which is comprised of avalanche photodiodes AD is produced using IC technology, particularly CMOS technology, and is integrated in a sensor chip ( 20 ) with Greiger-mode wiring, and; d) processing the signals, which are provided by the avalanche photodiode matrix, by means of a signal processing and evaluation device preferably integrated in the sensor chip.
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