<p>[PROBLEMS] To provide a high-precision method of determining the monoisotopic mass of protein and provide a relevant apparatus and program. [MEANS FOR SOLVING PROBLEMS] Use is made of a procedure comprising the step of fragmentizing one isotope of target protein and carrying out mass spectroscopic analysis thereof to thereby obtain mass spectrum pattern (1); the step of with respect to averagin product ions resulting from virtual fragmentization of averagin precursor ions corresponding to given-MW averagin multiple isotope peaks, carrying out virtual mass spectroscopic analysis to thereby obtain multiple mass spectrum patterns (2); the step of selecting mass spectrum pattern (2) with highest similarity to the mass spectrum pattern (1) from among the multiple mass spectrum patterns (2) and determining the difference between averagin precursor ion and monoisotopic mass; and the step of determining the monoisotopic mass of target protein from the difference.</p>
申请公布号
WO2006106724(A1)
申请公布日期
2006.10.12
申请号
WO2006JP306448
申请日期
2006.03.29
申请人
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCEAND TECHNOLOGY;TAKAHASHI, KATSUTOSHI;MOGUSHI, KAORU;TOYODA, MICHISATO