METHOD FOR DETERMINING NATIVE WOOD CONSTITUENTS USING VISIBLE-LIGHT RAMAN SPECTROMETRY

摘要

A method for the determination of solid native wood chemical constituents, such as total lignin content, Klason lignin, cellulose, hemicelluloses, and other carbohydrates, in native wood samples such as cores, strips, boards, logs, and in boreholes made in standing trees, with the aid of Raman spectrometry. The method typically comprises the steps of subjecting the sample to a monochromatic visible-light source, collecting the scattered light from the sample at either 180° back-scattering mode or 90° scattering mode for a set integration time over a predetermined range of wave numbers so as to produce Raman shift spectra, acquiring the spectral data over a pre-determined length of time, determining the peak scattering intensity, correlating by univariate or multivariate calibration between peak-intensity and/or spectral data with known values of solid native wood chemical composition and quantifying said spectra to previously determined calibration so as to determine solid native chemical compositions of unknown samples.