Ligation of synthetic zincfinger peptides to serial zincfingerproteins for specific detection of double stranded DNA (zincfinger probes)

摘要

Polyzinc finger molecule binder (I) comprises chemically synthesized peptides having C2H2-zinc finger motifs. In combination and in the form of multimers, the peptide can bind to any double stranded DNA. The section of DNA is freely selectable. Independent claims are also included for: (1) (I) synthesized through ligation of libraries (I)-(III) given in the specification; (2) library I of synthetized peptides with C2H2-zinc finger motifs; (3) library II of synthesized peptides with C2H2 zinc finger motifs; (4) library III of synthesized peptides with C2H2 zinc finger motifs, where the amino terminal of the peptides begin with unprotected cystein; (5) biochip on which (I) has been immobilized.