发明名称 |
NUCLEIC ACID SEQUENCING BY RAMAN MONITORING OF UPTAKE OF NUCLEOTIDES DURING MOLECULAR REPLICATION |
摘要 |
<p>The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction.</p> |
申请公布号 |
EP1706508(A2) |
申请公布日期 |
2006.10.04 |
申请号 |
EP20040815205 |
申请日期 |
2004.12.24 |
申请人 |
INTEL CORPORATION |
发明人 |
RAO, VALLURI;NEUBAUER, GABI;KIRCH, STEVEN;YAMAKAWA, MINEO;BERLIN, ANDREW |
分类号 |
C12Q1/68;B01L3/00;G01N21/05;G01N21/65 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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