发明名称 |
PRODUCTION OF HIGH TITER HELPER-FREE RETROVIRUSES BY TRANSIENT TRANSFECTION |
摘要 |
<p>A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.</p> |
申请公布号 |
EP0689601(B1) |
申请公布日期 |
2006.10.04 |
申请号 |
EP19940909777 |
申请日期 |
1994.02.22 |
申请人 |
THE ROCKEFELLER UNIVERSITY |
发明人 |
PEAR, WARREN, S.;NOLAN, GARRY, P.;SCOTT, MARTIN, L.;BALTIMORE, DAVID |
分类号 |
C12N15/09;C12N15/86;A61K39/00;A61K48/00;C12N5/10;C12N7/00;C12N15/867 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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