发明名称 |
Retinoid X receptor-interacting polypeptides and related molecules and methods |
摘要 |
Disclosed is a method for determining whether a test protein is capable of interacting with a retinoid X receptor protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including a retinoid X receptor protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the test protein covalently bonded to a gene activating moiety; and (b) determining whether the test protein increases expression of the reporter gene as an indication of its ability to interact with the retinoid X receptor protein. Also disclosed is purified DNA encoding retinoid X receptor-interacting proteins and the polypeptides expressed from such DNA.
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申请公布号 |
US7115711(B1) |
申请公布日期 |
2006.10.03 |
申请号 |
US19990365576 |
申请日期 |
1999.08.02 |
申请人 |
THE GENERAL HOSPITAL CORPORATION |
发明人 |
MOORE DAVID;SEOL WONGI;CHOI HUENG-SIK |
分类号 |
C07K14/00;G01N33/566;C07K14/47;C07K14/705;C12N5/10;C12N15/09;C12P21/02;C12Q1/68;C12R1/91 |
主分类号 |
C07K14/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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