HEPARIN/HEPAROSAN SYNTHASE FROM P.MULTOCIDA, SOLUBLE AND SINGLE ACTION CATALYSTS THEREOF AND METHODS OF MAKING AND USING SAME

摘要

The presently claimed and disclosed invention relates, in general, to single action, dual action and soluble heparin synthases and, more particularly, to single action, dual action and soluble heparin synthases obtained from Pasteurella multocida. The presently claimed and disclosed invention also relates to heparosan, heparin and heparin-like molecules provided by recombinant techniques and methods of using such molecules. The presently claimed and disclosed invention also relates to methods, and molecules produced according to such methods, for using the presently claimed and disclosed heparosan and/or heparin synthase for polymer grafting and the production of non­naturally occurring chimeric polymers incorporating stretches of one or more acidic GAG molecules, such as heparin, chondroitin, hyaluronan, and/or heparosan.