A NOVEL MODULAR TYPE II RESTRICTION ENDONUCLEASE, CspCI, AND THE USE OF MODULAR ENDONUCLEASES FOR GENERATING ENDONUCLEASES WITH NEW SPECIFICITIES

摘要

A novel restriction endonuclease and methods of making the same are obtainable from either Citrobacter species 2144 (NEB#1398) or the recombinant stain Escherichia coli (NEB#1554) which cleaves at nt sequence 5'-CAANNNNNGTGG-3'(SEQ ID NO:14) in double-stranded DNA molecules. The novel restriction endonuclease is a modular protein in which the specificity moiety is an independent module from the restriction-modification module.