FRAMEWORK-SHUFFLING OF ANTIBODIES

摘要

The present invention relates to methods of reengineering or reshaping
antibodies to reduce the immunogenicity of the antibodies, while maintaining
the immunospecificity of the antibodies for an antigen. In particular, the
present invention provides methods of producing antibodies immunospecific for
an antigen by synthesizing a combinatorial library comprising complementarity
determining regions (CDRs) from a donor antibody fused in frame to framework
regions from a sub-bank of framework regions. The invention also provides
method of producing improved humanized antibodies. The present invention also
provides antibodies produced by the methods of the invention.