Gene therapy for critical limb ischemia with wild type or mutant eNOS

摘要

The present invention provides novel methods of preventing, diagnosing, and treating Critical Limb Ischemia (CLI), using eNOS polypeptides and polynucleotides to modulate eNOS activity in cells. Wild-type and mutant eNOS polypeptides, and polynucleotides encoding such polypeptides, are provided for use in the methods of the present invention. The eNOS mutant polypeptides of the present invention have at least one mutation corresponding to a site in a functional domain of a mammalian eNOS that is phosphorylated in cells.