Dna fragment amplification method, reaction apparatus for amplifying dna fragment and process for producing the same

摘要

A reaction apparatus ( 10 ) includes a substrate ( 12 ) and a plurality of columnar members ( 14 ) formed on the substrate ( 12 ). Oligonucleotides for immobilization ( 16 ) having sequences complementary to sequences of both ends of a starting template DNA ( 18 ) is adhered on the surfaces of the substrate ( 12 ) and the columnar members ( 14 ). The starting template DNA ( 18 ) can be immobilized over the adjacent columnar members ( 14 ) by introducing the starting template DNA ( 18 ) under the elongated condition. PCR is conducted in such condition.