SEPARATION AND PURIFICATION OF ENZYMATIC PRODUCT AND HYDROLYZED COMPOSITION OBTAINED BY USING THE ENZYME OBTAINED BY SEPARATION AND PURIFICATION

摘要

PROBLEM TO BE SOLVED: To provide a method for separating enzymatic product based on theα-glucuronidase activity, the enzyme obtained thereby and the hydrolytic composition of polysaccharide originating from enzymatically hydrolyzed plant cell wall and/or tree sap by using the resultant enzyme. SOLUTION: An enzymatic product having hemicellulase activity andα-glucuronidase activity is subjected to the ultra-filtration treatment to separate the treated produce into the enzyme having theα-glucuronidase activity and the enzyme having noα-glucuronidase activity. Plant cell wall and/or tree sap polysaccharide are hydrolyzed with the enzyme having theα-glucuronidase activity or with the enzyme having noα-glucuronidase to obtain hydrolytic compositions. One example of the composition hydrolyzed with the enzyme having noα-glucuronidase activity comprises as a minimum unit, a tetramer in which one of glucuronic acid or 4-O-methyl glucuronate bonds to xylotriose with an average polymerization degree is 4 to 10. COPYRIGHT: (C)2006,JPO&NCIPI