发明名称 |
SKIN EQUIVALENT CULTURE |
摘要 |
Methods of forming soft connective tissue compositions such as skin equivalents, compositions made by the methods and their uses. In particular, a method of forming a connective tissue equivalent, comprising the steps of: (i) incubating collagen-producing cells in or on a support matrix; (ii) inducing and/or enhancing collagen production by the collagen-producing cells to form a collagenous construct and degradation and replacement of the support matrix; (iii) freeze-drying the construct; and (iv) re-populating the freeze-dried construct with collagen-producing cells and/or epithelial cells and/or endothelial cells and/or mesenchymal cells, thereby forming a connective tissue equivalent, wherein: (a) the collagen-producing cells are substantially fibroblasts; for example human neonatal dermal fibroblasts; (b) the support matrix is a provisional support matrix in which the support matrix is a fibrin matrix, for example formed by thrombin-mediated polymerisation of fibrinogen; and (c) as a result of the collagen production by the collagen-producing cells the provisional fibrin support matrix is digested by the cells and is replaced by collagen, thereby essentially replacing the provisional fibrin matrix with a collagen matrix synthesised in situ by the cells. |
申请公布号 |
WO2006097701(A2) |
申请公布日期 |
2006.09.21 |
申请号 |
WO2006GB00890 |
申请日期 |
2006.03.14 |
申请人 |
INTERCYTEX LIMITED;KEMP, PAUL;SHERING, DAVID;JOHNSON, PENNY;MARSHALL, DAMIAN |
发明人 |
KEMP, PAUL;SHERING, DAVID;JOHNSON, PENNY;MARSHALL, DAMIAN |
分类号 |
A61L27/38;A61L27/60;C12N5/071 |
主分类号 |
A61L27/38 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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