METHOD OF LESSENING NONSPECIFIC AMPLIFICATION FROM PRIMER DIMER

摘要

It is intended to provide a method of suppressing a nonspecific amplification reaction accompanying primer dimer formation in a nucleic acid amplification reaction characterized by comprising adding a substance capable of binding to the primer dimer to a sample. As the substance capable of binding to the primer dimer, use can be made of a substance capable of recognizing mismatch such as a mismatch-binding protein or a substance having a DNA recombination ability such as a DNA recombinase.