| 摘要 |
In an in-flow chemical reaction system (A) of the present invention, a sample solution ( 1 ) containing glycoprotein is injected into a reaction tube ( 7 ) in which an alkaline solution continuously flows. When a mixed solution of the sample solution and the alkaline solution reach a thermostat bath ( 6 ), reaction for cleaving a glycoside linkage takes place, thereby separating a carbohydrate chain from glycoprotein. From the solution containing the carbohydrate chain, alkali is removed by using an ion-exchange column ( 12 ), so that a fraction of a carbohydrate chain ( 14 ) is collected into a sample tube ( 13 ). In the in-flow chemical reaction system (A), reaction takes place in minutes, so that reaction time is significantly reduced compared to a conventional method. Thus, a glycoside linkage is readily cleaved with high precision in a short time, thereby separating carbohydrate from a glycoside-linkage-having compound.
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