DEVICE FOR INTRODUCING SUBSTANCE INTO CELL, CELL CLAMPING DEVICE AND FLOW PATH FORMING METHOD

摘要

A device for introducing a substance into a cell which can realize a high-efficient external substance introduction by means of electro-poration not depending on a cell size, a cell clamping device capable of clamping a cell at many locations, and a flow path forming method capable of efficiently forming a flow path. The device for introducing a substance into a cell (10a) comprises an insulating thin film (2) having a pore (1) and a pair of electrodes (6, 7) disposed on the opposite sides of the film (2) across the pore (1). When a cell (9) is fixed to the pore (1) and a pulse voltage is applied to between the electrodes (6, 7) with a space (5) filled with a fluid containing substance (4) to be introduced into the cell (9), a field concentration to a pore portion is used to destroy a cell membrane to thereby introduce the substance (4) into the cell (9).