发明名称 |
Ligand/binding partner bio-labeling systems |
摘要 |
The present invention relates to methods and compositions which utilize binding relationships between detector molecules and their ligands for labeling molecules of interest in vivo. A "target" molecule is linked to a detector molecule (such as a protein or a nucleic acid) and the function and/or location of the target molecule is monitored by binding the detector molecule its ligand, which carries a detectable label. In particular embodiments of the invention, an intracellular fusion protein comprising a target protein and a detector protein is bound to a membrane-permeable, fluorescently-labeled ligand of the detector protein, thereby providing an adjunct or alternative to Green Fluorescent Protein.
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申请公布号 |
US2006211007(A1) |
申请公布日期 |
2006.09.21 |
申请号 |
US20050219506 |
申请日期 |
2005.09.01 |
申请人 |
CORNISH VIRGINIA;SHEETZ MICHAEL;MILLER LARRY |
发明人 |
CORNISH VIRGINIA;SHEETZ MICHAEL;MILLER LARRY |
分类号 |
C12Q1/68;B65C;B65C1/00;C12Q1/00;C12Q1/02;C12Q1/26;G01N33/53;G01N33/573;G01N33/58 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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