发明名称 |
Methods and compositions comprising DNA damaging agents and p53 |
摘要 |
The present invention relates to the use of tumor suppressor genes in combination with a DNA damaging agent or factor for use in killing cells, and in particular cancerous cells. A tumor suppressor gene, p53, was delivered via a recombinant adenovirus-mediated gene transfer both in vitro and in vivo, in combination with a chemotherapeutic agent. Treated cells underwent apoptosis with specific DNA fragmentation. Direct injection of the p53-adenovirus construct into tumors subcutaneously, followed by intraperitoneal administration of a DNA damaging agent, cisplatin, induced massive apoptotic destruction of the tumors. The invention also provides for the clinical application of a regimen combining gene replacement using replication-deficient wild-type p53 adenovirus and DNA-damaging drugs for treatment of human cancer.
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申请公布号 |
US7109179(B2) |
申请公布日期 |
2006.09.19 |
申请号 |
US20040784538 |
申请日期 |
2004.02.23 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
ROTH JACK A.;FUJIWARA TOSHIYOSHI;GRIMM ELIZABETH A.;MUKHOPADHYAY TAPAS;ZHANG WEI-WEI;OWEN-SCHAUB LAURIE B. |
分类号 |
A61K31/713;C12N15/09;A61K9/08;A61K31/277;A61K31/40;A61K31/407;A61K31/4745;A61K31/505;A61K31/513;A61K31/70;A61K31/704;A61K31/7048;A61K31/711;A61K33/24;A61K35/76;A61K38/00;A61K38/17;A61K45/00;A61K48/00;A61K51/00;A61P35/00;C07K14/47;C07K14/82;C12N15/113;C12N15/861 |
主分类号 |
A61K31/713 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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