发明名称 |
CD19-SPEZIFISCHE UMGEZIELTE IMMUNZELLEN |
摘要 |
The present invention relates to a genetically engineered, CD19-specific chimeric T cell receptor and to immune cells expressing the chimeric receptor. The present invention also relates to the use of such cells for cellular immunotherapy of CD19<SUP>+</SUP> malignancies and for abrogating any untoward B cell function. The chimeric receptor is a single chain scFvFc:zeta receptor where scFvFc designates the extracellular domain, scFv designates the V<SUB>H </SUB>and V<SUB>L </SUB>chains of a single chain monoclonal antibody to CD19, Fc represents at least part of a constant region of an IgG<SUB>1</SUB>, and zeta represents the intracellular signaling domain of the zeta chain of human CD3. The extracellular domain scFvFc and the intracellular domain zeta are linked by a transmembrane domain such as the transmembrane domain of CD4. In one aspect, the chimeric receptor comprises amino acids 23-634 of SEQ ID NO:2. The present invention further relates to a method of making a redirected T cell expressing a chimeric T cell receptor by electroporation using naked DNA encoding the receptor. |
申请公布号 |
AT338124(T) |
申请公布日期 |
2006.09.15 |
申请号 |
AT20010273518T |
申请日期 |
2001.11.07 |
申请人 |
CITY OF HOPE |
发明人 |
JENSEN, MICHAEL, C.;FORMAN, STEPHEN;RAUBITSCHEK, ANDREW |
分类号 |
C12N15/09;A61K35/14;A61K35/28;A61K38/00;A61K38/20;A61K39/395;A61P35/00;A61P35/02;A61P37/02;A61P37/06;C07K14/705;C07K14/725;C07K16/28;C12N5/0783;C12N5/10;C12N15/12;(IPC1-7):C12N15/12 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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