ULTRA-LOW TEMPERATURE STORAGE TECHNIQUE FOR CULTURED PLANT CELLS

摘要

Ultra-low temperature storage is performed by employing pre-freezing technique using a cryoprotective solution comprising glycerin, sucrose and proline blended in a suitable manner. By using this method, procedures for the treatment of a cultured plant cell line (collection of cells by centrifugation/addition of a cryoprotective solution to the cells and resuspension of the cells in the solution/dispensation of the resuspended solution into Cryogenic vials) can be continued to operate at ordinary temperature for at least 120 minutes. Moreover, by using the cryoprotective solution in the cryoprotection treatment, ultra-low temperature storage of cultured plat cells can be achieved conveniently using an inexpensive, readily available cryogenic preservative container or EPS tube rack without using any expensive program freezer. The ultra-low temperature storage method using the cryoprotective solution is applicable to a wide range of plant species and a wide range of cultured cell lines.