发明名称 |
GLYCOENGINEERING IN MUSHROOMS |
摘要 |
The invention relates to the field of genetic engineering, more specifically to the field of production of glycoproteins, more specifically to the engineering of the glycosylation pattern of glycoproteins. Provided is a method for producing a glycoprotein in a recombinant host cell, comprising allowing expression of said glycoprotein in a basidiomycete host provided wi th N-acetylglucosamine : alpha-3-D-mannoside beta-1,2-N- acetylglucosaminyltransferase (GnT-I; EC 2.4.1.101) activity. Also provided is a basidiomycete host cell expressing GnT-I, preferably human GnT-I and use thereof for the manufacture of (mammalian) proteins carrying complex N- glycans, such as (monoclonal) antibodies.
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申请公布号 |
CA2600391(A1) |
申请公布日期 |
2006.09.14 |
申请号 |
CA20062600391 |
申请日期 |
2006.03.03 |
申请人 |
PLANT RESEARCH INTERNATIONAL B.V. |
发明人 |
ROUWENDAL, GERARD JOHAN ADOLPH;FLORACK, DIONISIUS ELISABETH ANTONIUS;LUGONES, LUIS GASTON;BOSCH, HENDRIK JAN |
分类号 |
C12P21/00 |
主分类号 |
C12P21/00 |
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