发明名称 |
METHOD FOR THE DISSOCIATION OF THE EXTRACELLULAR HAEMOGLOBIN MOLECULE OF ARENICOLA MARINA AND THE CHARACTERISATION OF THE PROTEIN CHAINS FORMING THE MOLECULE AND THE NUCLEOTIDE SEQUENCES CODING FOR SAID PROTEIN CHAINS |
摘要 |
The invention relates to a method of dissociating the extracellular haemoglobin molecule of annelids, e.g. Arenicola marina, which can be used to obtain the protein chains forming said molecule. The inventive method is characterised in that it comprises a step consisting in bringing an extracellular haemoglobin sample from annelids, e.g. Arenicola marina, into contact with at least one dissociating agent, e.g. a mixture containing dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP) hydrochloride or beta-mercaptoethanol and a dissociation buffer for a sufficient length of time in order to separate the protein chains from one another. |
申请公布号 |
WO2005037392(A3) |
申请公布日期 |
2006.09.14 |
申请号 |
WO2004FR02602 |
申请日期 |
2004.10.13 |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;UNIVERSITE PIERRE ET MARIE CURIE;ZAL, FRANCK;CHABASSE, CHRISTINE;ROUSSELOT, MORGANE;BAILLY, XAVIER |
发明人 |
ZAL, FRANCK;CHABASSE, CHRISTINE;ROUSSELOT, MORGANE;BAILLY, XAVIER |
分类号 |
C07K14/805;C12N15/12;C12P19/34;C12R1/85 |
主分类号 |
C07K14/805 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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