METHOD OF TARGETED AND COMPREHENSIVE SEQUENCING USING HIGH-DENSITY OLIGONUCLEOTIDE ARRAY

摘要

A method for targeted and comprehensive sequencing using high-density oligonucleotide array, comprising the steps of hybridizing nucleic acid from an investigative species with high-density oligonucleotide arrays of a related species, identifying the oligonucleotide probes that generate high hybridization signals, using the probes sequences to make PCR primers, amplifying heterologous genes by PCR with the gene specific PCR primers and an anchoring primer, and sequencing the PCR products.