发明名称 Specific and sensitive nucleic acid detection method
摘要 Method for the detection of a nucleic acid comprising the production of a plurality of amplificates of a section of this nucleic acid with the aid of two primers, one of which can bind to a binding sequence A of the nucleic acid and the other can bind to a binding sequence C' which is complementary to a sequence C which is located in the 3' direction from A and does not overlap with A, contacting the amplificates with a probe having a binding sequence D which can bind to a sequence B which is located between the sequences A and C or to the complement thereof, and detecting the formation of a hybrid of the amplificate and probe where the sequence located between the binding sequences A and C contains no nucleotides that do not belong to the binding sequence D of the probe or its complement D'.
申请公布号 US7105318(B2) 申请公布日期 2006.09.12
申请号 US20020322138 申请日期 2002.12.17
申请人 ROCHE DIAGNOSTICS GMBH 发明人 KESSLER CHRISTOPH;HABERHAUSEN GERD;BARTL KNUT;ORUM HENRIK
分类号 C12N15/09;C12P19/34;C07H21/02;C07H21/04;C12Q1/68 主分类号 C12N15/09
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