| 摘要 |
Using a protein associated with myc (PAM) (I) or its functional fragments or derivatives for the preparation of pharmaceutical compounds, for modulation of pain and for identifying compounds that modulate pain, is new. An independent claim is also included for PAM or its functional fragment for the use as medicament for the prevention or treatment of pain. ACTIVITY : Analgesic. MECHANISM OF ACTION : Inhibits adenylyl cyclase activity (claimed). In vitro analysis of the activity of protein associated with myc (PAM) in inhibiting adenylyl cyclase activity was carried out as follows. The sense or antisense oligonucleotides of PAM were infused through a catheter at a flow rate of 0.05-0.1 mu l/minute for 100 hours using a microinfusion pump, into rats. Within 15 minutes after stopping the infusion, the formalin test was performed. For induction of an inflammation, Zymosan A was injected subcutaneously. Rats were killed by cardiac puncture under deep isoflurane anesthesia 24-96 hours after Zymosan injection. The lumbar spinal cord and dorsal root ganglia were excised, and subjected to analysis. The result indicated an inhibition of adenylyl cyclase activity in the spinal cord lysates of the sense strand treated rats, and an increase in adenylyl cyclase activity in the antisense treated rats. |
