СПОСОБ ПОЛУЧЕНИЯ СЕКРЕТОРНОГО ИММУНОГЛОБУЛИНА А ИЗ БИОЛОГИЧЕСКОЙ ЖИДКОСТИ ЖИВОТНЫХ

摘要

FIELD: veterinary science. ^ SUBSTANCE: the present innovation deals with obtaining secretory immunoglobulin A (S-IgA) applied for evaluating immune status in animals. The method deals with biochemical treatment of animals' biological liquid, separating a target product due to gel-filtration followed by freezing, moreover, biological liquid should be centrifuged at 1000-1700 g for about 15-30 min followed by dialysis against 0.02 M Tris-HCL pH 8.0-8.2 for about 1.5-2.5 h and gel-filtration upon Sephacryl S-400 with 0.015-0.02 M Tris-HCL buffer at pH being 8.0-8.2 and with 0.4-0.9 M sodium chloride due to separating the second fraction of proteins under the control of immunoelectrophoresis at applying antisera to animal proteins, as for the target product it should be obtained due to concentrating the fractions of proteins in polyethylene glycol-40000 to increase the content of immunoglobulin A in the target product by 3-6-fold in comparison to its content in initial biological liquid in animals. The innovation provides higher output and quality of the target product and more accelerated rate of method's implementation. ^ EFFECT: higher efficiency. ^ 3 ex