| 摘要 |
PROBLEM TO BE SOLVED: To provide a method of genotyping, enabling a low-cost assay without fluorescent oligonucleotides and enabling a high success rate. SOLUTION: The method for determining a base at a particular nucleotide polymorphism site comprises amplifying the nucleic acid sample comprising two kinds of forward primers complementary to a first base and a secondary base at the particular nucleotide polymorphism site, and exhibiting different melting temperatures, a reverse primer complementary to a downstream sequence of the particular nucleotide polymorphism site, and a DNA polymerase, measuring the melting temperature of the amplification reaction product, and determining the base at the particular nucleotide polymorphism site by the melting temperature. COPYRIGHT: (C)2006,JPO&NCIPI
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