| 摘要 |
In some embodiments, the present disclosure provides admixtures and methods for PCR amplification of a target polynucleotide in the presence of forward and reverse amplification primers and in which the occurrence of primer-dimers is decreased by including a dummy target polynucleotide in the PCR reaction mix. The dummy target polynucleotide is simultaneously amplified by the amplification primers and is designed to form a less stable hybrid with an amplification primer as compared to the hybrid formed between the amplification primer and the target polynucleotide. Kits for carrying out the various methods are also provided.
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